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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Herstatin (HER-2, neu Receptor Tyrosine Kinase, c-erbB-2)

Anti -Herstatin (HER-2, neu Receptor Tyrosine Kinase, c-erbB-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Herstatin (HER-2/neu Receptor Tyrosine Kinase) (HER-2/neu, c-erbB-2) oncogene causes tumorigenesis and malignant progression of human cancers. The HER-2/neu oncogene encodes a receptor tyrosine kinase that is overexpressed in several types of human cancer. In breast cancer, overexpression of HER-2 occurs in 30% of the cases where it confer more aggressive tumor growth and poor outcome.
Catalog #H2034-30
ApplicationsSuitable for use in Immunohistochemistry and Immunoblot. Other applications not tested.
Recommended DilutionImmunohistochemistry: Detects Herstatin in stroma of HER-2 positive breast tissue.
Immunoblot Analysis: 0.5-2ug/ml detects Herstatin in lysates from Cos-7 cells transfected with Herstatin. 20ug of 293 cell lysates from nontransfected cells and Herstatin/HER-2neu co-transfected cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Herstatin, (1ug/ml). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4i42
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenHis-tagged fusion protein corresponding to amino acids 340-418 of human Herstatin.
SpecificityRecognizes human Herstatin (Mr ~60kD) and Herstatin aggregates (Mr ~150kD).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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