| Herstatin (HER-2/neu Receptor Tyrosine Kinase) (HER-2/neu, c-erbB-2) oncogene causes tumorigenesis and malignant progression of human cancers. The HER-2/neu oncogene encodes a receptor tyrosine kinase that is overexpressed in several types of human cancer. In breast cancer, overexpression of HER-2 occurs in 30% of the cases where it confer more aggressive tumor growth and poor outcome. |
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| Catalog # | H2034-30 |
| Applications | Suitable for use in Immunohistochemistry and Immunoblot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: Detects Herstatin in stroma of HER-2 positive breast tissue. |
| Immunoblot Analysis: 0.5-2ug/ml detects Herstatin in lysates from Cos-7 cells transfected with Herstatin. 20ug of 293 cell lysates from nontransfected cells and Herstatin/HER-2neu co-transfected cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Herstatin, (1ug/ml). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 4i42 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, before the addition of glycerol to 30%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | His-tagged fusion protein corresponding to amino acids 340-418 of human Herstatin. |
| Specificity | Recognizes human Herstatin (Mr ~60kD) and Herstatin aggregates (Mr ~150kD). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
