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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -HES1 (Hairy Family Helix-loop-helix Transcription Factor)

Anti -HES1 (Hairy Family Helix-loop-helix Transcription Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
HES1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. HES1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and HES1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
Catalog #H2034-35
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsWestern Blot (ECL): 1:200-1:1000
Immunohistochemistry (Frozen sections): 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlHuman neural stem cells, Raji cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa24-35, TPDKPKTASEHR, of human HES1 (NP_005515).
SpecificityRecognizes human HES1 (Hairy family) at ~29kD. Species Crossreactivity: rodent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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