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You are here:Home » Antibodies » Abs to Chromatin Proteins » Anti -Heterochromatin Protein 1, beta (HP1-beta, Chromobox Homolog 1, CBX1, Heterochromatin Protein p25 beta, HP1Hs-beta, M31, Modifier 1 Protein, MOD1, p25beta)

Anti -Heterochromatin Protein 1, beta (HP1-beta, Chromobox Homolog 1, CBX1,
Heterochromatin Protein p25 beta, HP1Hs-beta, M31, Modifier 1 Protein, MOD1, p25beta)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Catalog #H2034-51G
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other Applications not tested.
Recommended DilutionsELISA: 1:32,000
Immunohistochemistry (paraffin): 2-3ug/ml
Western Blot: 0.3-1.0ug/ml
Optimal dilutions to be determined by the researcher
Positive ControlLysates of A431, HeLa and HepG2
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See H2034-51G-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide, C-QSQKTAHETDKSE, from the internal region of human CBX1.
SpecificityRecognizes human CBX1 at ~25kD. Species sequence homology: mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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