| Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1alpha, HP1beta and HP1gamma). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. |
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| Catalog # | H2034-52 |
| Applications | Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:500 detected HP1gamma on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF and probed with diluted H2034-52. Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system. |
| Immunofluorescence: 1:500-1:5000 |
| Immunohistochemistry: 1:500-1:5000 |
| ELISA: 1:500-1:5000 |
| Optimal dilution determined by the researcher. |
| *Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-14C: IgG, Fc (HRP) Pab Gt x Mo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo |
| Recommended Control | HeLa cell lysate, NIH/3T3, C6 & COS cells |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q2290 |
| Host | Mouse |
| Source | Mouse |
| Concentration | Not Determined |
| Form | Supplied as a liquid. No preservative added. |
| Purity | Ascites |
| Immunogen | Recombinant mouse HP1gamma. |
| Specificity | Recognizes mouse Heterochromatin protein-1 gamma (HP1g) at ~22kD. Species Crossreactivity: human, rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
