| Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1alpha, HP1betta and HP1gamma). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. |
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| Catalog # | H2034-52C |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.01-0.1ug/ml Detects HP1g protein in acid-extracted HeLa cell lysates. |
| Immunohistochemistry: Detects HP1g positive cells in breast tissue. |
| Immunoprecipitation (Chromatin): Immunoprecipitate HP1g from nuclear extracts and formalin-cross-linked chromatin. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | HeLa acid extracts, HEK 293 nuclear extracts |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5H14 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.175M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | GST fusion protein corresponding to aa17-173 from human Heterochromatin Protein 1, gamma. |
| Specificity | Recognizes human HP1g protein, MW ~25kD. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
