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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum IH IF

Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach.
Catalog #	H5080-04
Applications	Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Frozen and paraffin sections): 1:1500-1:2000 (PAP).
	Immunofluorescence: 1:300-1:600 with overnight incubation at 4°C.
	Optimal dilutions to be determined by the researcher.
Recommended Positive Control	Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not Determined
Form	Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O Further dilutions should be made in PBS, 1% BSA, 0.1% sodium azide.
Purity	Serum
Immunogen	Histamine, conjugated to BSA
Specificity	Recognizes Histamine. Absorption with 10-100ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not. Species Crossreactivity: human and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.