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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum IH IC

Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach.
Catalog #	H5080-06
Applications	Suitable for use in Immunohistochemistry and Immunocytochemistry. Not suitable for use on samples fixed with aldehydes. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:1000-1:4000 on brain and stomach using PAP or ABC. Suggested fixative is 4% EDAC or EDAC + PFA.
	Immunocytochemistry: 1:200-1:5000 (cultured cells 2H3 mastocytoma)
	Note: Preabsorption of the antiserum with a histamine-BSA or histamine-ovalbumin conjugate (1-100ug/ml) completely abolished non-specific binding.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Serum.
Immunogen	Histamine conjugated to KLH with EDAC.
Specificity	Recognizes Histamine. No reactivity to L-histadine containing peptides. Species Crossreactivity: Vertebrates and invertebrates (human, rat, mouse, frog, fish, mollusks, and flatworm).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.