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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Histidine Tag

Anti -Histidine Tag

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Catalog #H5099-13
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Frozen sections
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-65W: IgG, F(abí)2 (HRP) Pab Rb xMo
I1904-66C: IgG, F(abí)2X-adsorbed (HRP) Pab Rb xMo
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9A172
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPAX6 transcription factor linked to histidine tag
SpecificityRecognizes Histidine tag proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Tracks the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins.
In WesternBlot of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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