| Catalog # | H5099-13 |
| Applications | Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry: Frozen sections |
| Western Blot: 1:500-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo |
| I1904-66C: IgG, F(ab’)2X-adsorbed (HRP) Pab Rb xMo |
| Hybridoma | Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 9A172 |
| Host | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | PAX6 transcription factor linked to histidine tag |
| Specificity | Recognizes Histidine tag proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Tracks the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins. |
| In WesternBlot of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
