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You are here:Home » Antibodies » Antibodies-Epitope Tags » Anti -His-Tag (6-Histidine) (AP)

Anti -His-Tag (6-Histidine) (AP)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or C-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Tags enable a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions. This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins. Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
Catalog #H5099-15A
ApplicationsSuitable for use in ELISA, Dot Blot, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1;50,000
Immunohistochemistry: 1:200-1:2000
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C after receipt as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Clone TypeMonoclonal
Clone No3H27
FormSupplied as a liquid in 0.09% sodium azide. Labeled with alkaline phosphatase (AP).
PurityPurified by Protein A/G affinity chromatography
ImmunogenHis-tag-fusion protein.
SpecificityRecognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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