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You are here:Home » Antibodies » Abs to Biochemicals » Anti -His-Tag (6-Histidine)

Anti -His-Tag (6-Histidine)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
The His-tagged expression system is widely used in the production of recombinant proteins. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Catalog #H5099-16
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Suitable for tracking the purification of proteins expressed in bacteria, yeast or insect cells. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:1000
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-65C: IgG, H&L (HRP) Pab Rb x Mo
I1904-65H: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Mo
I1904-65N: IgG (HRP) Pab Rb x Mo
I1903-07E: IgG (HRP) Pab Rb x Mo
I1904-67R: IgG H&L, X-adsorbed (HRP) Pab Rb x Mo
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
SourceTissue cuture supernatant
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2201
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPAX6 transcription factor linked to 6-histidine tag (HHHHHH).
SpecificityRecognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Suitable for tracking the purification of proteins expressed in bacteria, yeast or insect cells. , Recognizes all His tagged proteins so far tested. In Western Blot of bacterial extracts, do not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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