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You are here:Home » Antibodies » Antibodies-Biochemicals » Anti -His-Tag (6-Histidine) (Biotin)

Anti -His-Tag (6-Histidine) (Biotin)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion.
Catalog #H5099-16A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:500-1:1000; Tested using histidine tagged ubiquitin.
Western Blot: 1:1000; Tested using His-tagged recombinant protein control. H5099-16A has been shown not to crossreact with any endogenous products when using bacterial extracts. Some crossreactivity may be seen with extracts of insect or mammalian cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentS7973-97B: Streptavidin (HRP)
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3H2201
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenPAX6 transcription factor linked to histidine tag.
SpecificityRecognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Reacts against all His tagged proteins so far tested. May be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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