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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E IH IC

Catalog #	H5100-01
Applications
	Suitable for use in ELISA Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended dilutionImmunohistochemistry	1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Specificity	Using a conjugate L-Histidine-Pc, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
Compound	Cross-reactivity ratio:
	L-Histidine-G-BSA 1
	Histamine-G-BSA 1/>100,000
	L-Carnosine-G-BSA 1/>100,000
(a)	L-Histidine-G-BSA concentration/other conjugated aminoacid concentration at half displacement;
	G = Glutaraldehyde, BSA : Bovine Serum Albumin.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid. No preservative added.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic L-Histidine conjugated to protein carrier (Pc) (L-Histidine-Glutaraldehyde-BSA)
Specificity	Recognizes protein-conjugated Histidine. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.