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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified B IP

In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histone 2 is rich in lysine.
Catalog #	H5109-01
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Sheep
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4 before the addition of glycerol to 40%. No preservative added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact calf Histone 2B complexed to rRNA. MW of antigen: 13.8kD
Specificity	Recognizes bovine Histone 2B. Species crossreactivity: All other DNA containing species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.