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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IP

In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histone-III is moderately rich in arginine and contains cysteine.
Catalog #	H5109-10
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunoprecipitation: 2.5-10.0ug/mL
	Western Blot: Relative MW 15.3kD
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Sheep
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact calf H-III complexed to rRNA
Specificity	Recognizes human Histone 3. Species crossreactivity: Most other DNA containing species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.