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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IP IF

Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity.
Catalog #	H5110
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunoflourescence. Other applications not tested.
Recommended Dilution	Immunoprecipitation: 1:3000-1:15,000
	Optimal dilutions to be determined by the researcher.
Molecular Weights of Histones	Lysine Rich (H1, f1): ~ 21,500
	Slightly Lysine Rich (H2a, f2a2): 14,004
	Slightly Lysine Rich (H2b, f2b): 13,774
	Arginine Rich (H3, f3): 15,324
	Arginine Rich (H4, f2a1): 11,282
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Histone 1, calf whole protein complexed to ribosomal RNA.
Specificity	Recognizes bovine Histone-H-1: 100%. No reaction with Histone-H-2b , Histone-H-3 or Histone-H-4. Very low crossreactivity with Histone-H-core.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.