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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limited number of types of fractions (see below) with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.
Catalog #	H5110-01A
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (formalin/paraffin): 2-4ug/ml. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues
	Optimal dilutions to be determined by the researcher.
Positive Control	Tonsil or lymph node
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	0.N.331
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See Cat# H5110-01AX.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Nuclei of myeloid leukemia biopsy cells.
Cellular Localization	Nuclear
Specificity	Recognizes human histone H1.
Molecular Weights of Histones
Lysine Rich (H1, f1)	~ 21,500
Slightly Lysine Rich (H2a, f2a2)	14,004
Slightly Lysine Rich (H2b, f2b)	13,774
Arginine Rich (H3, f3)	15,324
Arginine Rich (H4, f2a1)	11,282
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.