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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H1

Anti -Histone H1

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP IH
Catalog #H5110-01B
In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins call Histones. The recurring positive charges of the histones form electrostataic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Molecular weight 21kD.
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:300
Immunoprecipitation: 1:20
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by Protein G affinity chromatography.
ImmunogenIntact calf Histone H1 complex to rRNA.
SpecificityRecognizes bovine Histone H1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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