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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H1

Anti -Histone H1

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependent manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30nm chromatin fiber.
Catalog #H5110-01E
ApplicationsSuitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: Speckled staining is observed in the nucleus of normal and malignant cells.
Western Blot: 1:100. In blots of rat brain homogenate a duplet is observed at 27-29kD consistent with Histone H1.
Immunohistochemistry (Frozen, paraffin): Acetone or PFA fixation of frozen sections is recommended.
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10D158
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenNuclei from myeloid leukamia biopsy cells.
SpecificityRecognizes human Histone H1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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