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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IP IF

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Catalog #	H5110-02C
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Glycerol free.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Calf thymus intact Histone H1 (MW 23kD) complexed to rRNA.
Cellular Localization	Nuclear.
Specificity	Recognizes bovine Histone H1. Specifically binds to the Histone H1 to protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. No crossreactivity was observed with other Histone core proteins H2a, H2b, H3 or H4. Species Crossreactivity: all mammals. Does not crossreact with the chicken linker histones H1 or H5.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.