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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H1, phosphorylated

Anti -Histone H1, phosphorylated

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IP IH IC
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Catalog #H5110-03C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgMk
Clone No9H243
HostMouse
SourceBovine
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySupernatant
ImmunogenKLH-conjugated bovine nuclear extract.
SpecificityRecognizes phosphorylated Histone H1 at ~32kD. Species Crossreactivity: human, mouse, bovine. Broad species cross-reactivity is expected, based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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