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You are here:Home » Antibodies » Abs to Histones » Anti -H1 histone family, member 2, phosphorylated (Ser27) (H1.B, histone cluster 1, H1c, H1.2, H1F2, Histone H1.2, Histone H1d, MGC3992)

Anti -H1 histone family, member 2, phosphorylated (Ser27) (H1.B, histone cluster 1, H1c,
H1.2, H1F2, Histone H1.2, Histone H1d, MGC3992)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Catalog #H5110-03D
ApplicationsSuitable for use in Western Blot and Luminex. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml. Detected H5110-03D in untreated and cocemid-treated HeLa acid extract.
Optimal dilutions to be determined by the researcher.
Positive ControlUntreated and colcemid-treated HeLa acid extract.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic linear peptide corresponding to the region at and around phosphorylated serine 27 of histone H1.B (KLH).
SpecificityRecognizes phosphorylated serine 27 at and around the N-terminal region of human histone H1.B at ~35kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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