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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H2A.X, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX)

Anti -Histone H2A.X, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X,
H2AFX, H2AX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage.
H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways
Catalog #H5110-03K
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml detected phosphorylated histone H2A.X (Ser139) in RIPA lysates from Jurkat cells treated with staurosporine and in acid-extracted proteins from gamma-irradiated human astrocytoma SF268 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months after receipt at -20°C from date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.014 PBS, pH 7.6, 0.07% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide Corresponding to aa134-142 (CKATQA[pS]QEY) of human histone H2A.X (KLH coupled). The immunizing sequence has 8 identical amino acids in yeast and mouse.
SpecificityRecognizes human Histone H2A phosphorylated at Ser139, MW 15kD. Species Crossreactivity: Broad species crossreactivity expected based on conservation of sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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