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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X, phosphorylated (Ser139) (H2A histone family member X, H2A.FX, H2A/X, H2AFX, H2AX)

Anti -Histone H2A.X, phosphorylated (Ser139) (H2A histone family member X, H2A.FX, H2A/X,
H2AFX, H2AX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #H5110-03K
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml detected phosphorylated histone H2A.X (Ser139) in RIPA lysates from Jurkat cells treated with staurosporine and in acid-extracted proteins from gamma-irradiated human astrocytoma SF268 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide Corresponding to aa134-142 (CKATQA[pS]QEY) of human histone H2A.X (KLH coupled). The immunizing sequence has 8 identical amino acids in yeast and mouse.
SpecificityRecognizes human Histone H2A phosphorylated at Ser139, Mr 15kD. Species Crossreactivity: Broad species crossreactivity expected based on conservation of sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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