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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X (H2AX)

Anti -Histone H2A.X (H2AX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage.
H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways.
Catalog #H5110-03L
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-1:25,000
Immunofluorescence: 1:400-1:800
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsI1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb
Recommeded Negative ControlH5110-03S: Histone H2A.X, Control Peptide, Human
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in TRIS-citrate/phosphate, 0.09% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region within the C-terminus of human H2AX.
SpecificityRecognizes human histone H2AX.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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