| H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage. |
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| H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways. |
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| Catalog # | H5110-03L |
| Applications | Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:5000-1:25,000 |
| Immunofluorescence: 1:400-1:800 |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Reagents | I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb |
| Recommeded Negative Control | H5110-03S: Histone H2A.X, Control Peptide, Human |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in TRIS-citrate/phosphate, 0.09% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to a region within the C-terminus of human H2AX. |
| Specificity | Recognizes human histone H2AX. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
