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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X (H2A Histone Family Member X, H2AFX, H2A.FX, H2AX, H2A.X, H2A/X)

Anti -Histone H2A.X (H2A Histone Family Member X, H2AFX, H2A.FX, H2AX, H2A.X, H2A/X)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase. Histone H2A.X mRNA contains polyA addition motifs and a polyA tail, conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. Two forms of H2A.X mRNA, consistent with the two sets of processing signals were found in proliferating cell cultures. One, about 1600 bases long, contains polyA; the other, about 575 bases long, lacks polyA. The short form behaves as a replication type histone mRNA, decreasing in amount when cell cultures are incubated with inhibitors of DNA synthesis, while the longer behaves as a basal type histone mRNA. human Histone H2A.X gene is mapped on chromosomal region 11q23.2-q23.3.
Catalog #H5110-03M
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlHUVEC cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
SpecificityRecognizes human Histone H2A.X. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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