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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX)

Anti -Histone H2A.X (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
Catalog #H5110-03N
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:5000-1:15,000 detects a band at ~25kD representing monoubiquinated H2AX protein and at~14kD representing H2AX.
Immunoprecipitation: 5-20ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman 293T nuclear extracts
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate pH 7.5, 0.09% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of human Histone H2AX (GeneID 3014).
SpecificityRecognizes human H2AX. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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