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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX)

Anti -Histone H2A.X (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
Catalog #H5110-03P
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormTris-citrate/phosphate [pH 7-8] containing 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenImmunogen was a synthetic peptide, which represented a portion of the C-terminus of human histone H2AX (LocusLink ID 3014).
SpecificityThis antibody is specific to human H2AX. Species Crossreactivity: can be used to detect H2AX in human and mouse. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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