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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2AX (Histone H2A.x, H2a/x, H2AX, H2AFX)

Anti -Histone H2AX (Histone H2A.x, H2a/x, H2AX, H2AFX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
Catalog #H5110-03Y
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2500
Immunofluorescence: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to human H2AX at C-terminus.
SpecificityRecognizes human H2AX. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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