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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser10) (Alexa FluorŪ 488)

Anti -Histone H3, phosphorylated (Ser10) (Alexa FluorŪ 488)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IC FC IF
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8).
Catalog #H5110-04B
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunofluorescence (IC): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Product sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 2mg/ml BSA. Labeled with Alexa Fluor® 488 (F/P ratio: 2-6).
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3 (KLH).
SpecificityRecognizes endogenous levels of human histone H3 only when phosphorylated at serine 10 (Mr 17kD). Does not crossreact with other phosphorylated histones or with acetylated histones. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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