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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X (H2A histone family member X, H2A.FX, H2A/X, H2AFX)

Anti -Histone H2A.X (H2A histone family member X, H2A.FX, H2A/X, H2AFX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Catalog #H5110-08A1
ApplicationsSuitable for use in Western Blot and Peptide Inhibition analysis. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:2000-1:10,000 dilution of this lot detected histone H2A.X in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant Histone H2A.
Peptide Inhibition Analysis: 1 and 10uM of the immunizing histone H2A.X peptide abolished detection of histone H2A.X by this lot in immunoblot analysis of HeLa acid extracts.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide and 30% glycerol.
PuritySerum
ImmunogenKLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to aa121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.
SpecificityRecognizes human histone H2A.X, Mr 15kD. A band at Mr 100kD was also observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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