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Anti -Histone H2A.X (H2A histone family member X, H2A.FX, H2A/X, H2AFX)
Anti -Histone H2A.X (H2A histone family member X, H2A.FX, H2A/X, H2AFX)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B |
|
| Catalog # | H5110-08A1 | | Applications | Suitable for use in Western Blot and Peptide Inhibition analysis. Other applications not tested. | | Recommended Dilution | Immunoblot Analysis: A 1:2000-1:10,000 dilution of this lot detected histone H2A.X in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant Histone H2A. | | Peptide Inhibition Analysis: 1 and 10uM of the immunizing histone H2A.X peptide abolished detection of histone H2A.X by this lot in immunoblot analysis of HeLa acid extracts. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid containing 0.05% sodium azide and 30% glycerol. | | Purity | Serum | | Immunogen | KLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to aa121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse. | | Specificity | Recognizes human histone H2A.X, Mr 15kD. A band at Mr 100kD was also observed. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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