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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H2A.X, phosphorylated (S139) (Biotin) (H2A histone family member X, H2A.FX, H2A/X, H2AFX, H2AX)

Anti -Histone H2A.X, phosphorylated (S139) (Biotin) (H2A histone family member X, H2A.FX,


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Catalog #H5110-08B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-1ug/ml detects phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine. Untreated, and staurosporine-treated Jurkat cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-08B (0.5ug/ml). Proteins were visualized using a streptavidin, horseradish peroxidase conjugate and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsS7973-97A: Streptavidin (HRP)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2T14
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 30%. Labeled with Biotin. Biotin
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X, KLH-conjugated.
SpecificityRecognizes human Histone H2A.X phosphorylated at Ser139, Mr 14kD. Species sequence homology: A broad species crossreactivity is expected based on conservation of sequence homology. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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