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Anti -Histone H2A.X, phosphorylated (S139) (Biotin) (H2A histone family member X, H2A.FX, H2A/X, H2AFX, H2AX)
Anti -Histone H2A.X, phosphorylated (S139) (Biotin) (H2A histone family member X, H2A.FX,
H2A/X, H2AFX, H2AX)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Catalog # | H5110-08B | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Immunoblot Analysis: 0.5-1ug/ml detects phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine. Untreated, and staurosporine-treated Jurkat cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-08B (0.5ug/ml). Proteins were visualized using a streptavidin, horseradish peroxidase conjugate and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Reagents | S7973-97A: Streptavidin (HRP) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2T14 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 30%. Labeled with Biotin. Biotin | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X, KLH-conjugated. | | Specificity | Recognizes human Histone H2A.X phosphorylated at Ser139, Mr 14kD. Species sequence homology: A broad species crossreactivity is expected based on conservation of sequence homology. The immunizing sequence has 8 identical amino acids in yeast and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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