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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X, phosphorylated (Ser139) (H2A histone family member X, H2A.FX, H2A/X, H2AFX)

Anti -Histone H2A.X, phosphorylated (Ser139) (H2A histone family member X, H2A.FX, H2A/X,
H2AFX)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC IF
H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage.
H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways.
Catalog #H5110-08C
ApplicationsSuitable for use in Western Blot, Immunofluorescence (2), Chromatin Immunoprecipitation (2) and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot Analysis: 0.5-1ug/ml detects phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5uM staurosporine. Untreated, and staurosporine-treated Jurkat cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-08C (0.5ug/ml). Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system.
Immunocytochemistry: 2ug/ml detects phosphorylated histone H2A.X (Ser139) in HeLa cells treated with 0.5uM staurosporine for 4-6 hours.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Positive ControlUV-treated 293 cell exxtracts, UV-treated HeLa cell extracts, breast cancer tissue.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2T14
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide and 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A.X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse. MW: 17kD
SpecificityRecognizes human Histone H2A.X phosphorylated at Ser139, Mr 15kD. A broad species crossreactivity is expected based on conservation of sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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