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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A, acidic patch, aa88-97

Anti -Histone H2A, acidic patch, aa88-97

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
Catalog #H5110-08J
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000 -1:5000. Detects Histone H2A (acidic patch) in acid extracts from Hela cell lysates.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Postive ControlHela cell extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa 88-97, IRNDEELNKL-C of human Histone H2A, conjugated to KLH (NP_003508).
SpecificityRecognizes human Histone H2A, at ~14kD. Species Crossreactivity: mouse, rat, chicken and yeast. Broad species crossreactiviy is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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