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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A

Anti -Histone H2A

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Histone H2A is the core component of the nucleosome. It is involved in nucleosome wrapping and compacting of DNA into chromatin, limiting DNA’s accessibility to the cellular machinery. Histones thereby play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, called histone code, and nucleosome remodeling.
Catalog #H5110-08M1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the residues near carboxyl terminal of human histone H2A protein (Swiss Prot: Q16777).
SpecificityRecognizes endogenous levels of human histone H2A protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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