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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IH

The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Catalog #	H5110-08M2
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Immunoprecipitation: 10-20ug/ml
	Immunohistochemistry: 10-30ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa cell nuclear extract
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to the N-terminus of human histone H2A
Specificity	Recognizes human Histone H2A at 14kD. Species Crossreactivity: mouse, rat and chicken. Does not react with other histones.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.