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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A

Anti -Histone H2A

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Catalog #H5110-08M2
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Immunoprecipitation: 10-20ug/ml
Immunohistochemistry: 10-30ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell nuclear extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to the N-terminus of human histone H2A
SpecificityRecognizes human Histone H2A at 14kD. Species Crossreactivity: mouse, rat and chicken. Does not react with other histones.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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