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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A

Anti -Histone H2A

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Catalog #H5110-08P
Recommended DilutionsELISA: 1:1000
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlJurkat or PBMC
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography
ImmunogenSynthetic peptides containing aa1-15, MSGRGKQGGKARAKA and 81-96, PRHLQLAIRNDEELNK of human Histone H2A (NP_066390.1).
SpecificityRecognizes human Histone H2A at ~14kD.
Species CrossreactivityMouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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