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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A, acetylated (Lys5, Lys9, Lys13, Lys15)

Anti -Histone H2A, acetylated (Lys5, Lys9, Lys13, Lys15)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Catalog #H5110-08Q
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:500-1:10,000 dilution of this lot detected acetylated Histone H2A in acid extracted proteins from sodium butyrate treated HeLa cells. Unacetylated recombinant Histone H2A was not detected.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide and 30% glycerol.
PuritySerum depleted of non-acetyl specific antibodies
ImmunogenKLH-conjugated, synthetic peptide containing (..GRG[AcK]QGG[AcK]ARA[AcK]A[AcK]TRS..), in which AcK corresponds to acetyl-lysine residues 5, 9, 13 and 15 of human Histone H2A.
SpecificityRecognizes acetylated Histone H2A, Mr ~14kD. Species Crossreactivity: Human. Predicted to crossreact with mouse and rat based on sequence conservation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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