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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A, acetylated (Lys5)

Anti -Histone H2A, acetylated (Lys5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Catalog #H5110-08Q1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:5000-1:50,000 dilution of this lot detected increased levels of acetylated Histone H2A in sodium butyrate treated HeLa cell acid extracted proteins when compared with acid extracts from untreated HeLa cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide and 30% glycerol.
PurityWhole rabbit antiserum depleted of non-acetyl specific antibodies
ImmunogenKLH-conjugated, synthetic peptide (..RGAcKQG…) in which AcK corresponds to acetyl lysine at position 5 of human Histone H2A.
SpecificityRecognizes acetylated Histone H2A, Mr ~14kD. An additional band of Mr 22kD was also detected at higher dilutions, and is likely the ubiquitinated form of Histone H2A. Species Crossreactivity: Human. Broad species crossreactivity expected due to conserved sequence of the immunogen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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