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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A, acetylated (Lys5) (H2A, HIST1H2A, H2AFM)

Anti -Histone H2A, acetylated (Lys5) (H2A, HIST1H2A, H2AFM)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces
monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs
after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).
Catalog #H5110-08Q1C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry andImmunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100,000
Immunoprecipitation: 1:70
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H373
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant.
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human Histone H2A..
SpecificityRecognizes human Histone H2A acetylated at Lysine-5. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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