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Anti -Histone H2A, acetylated (Lys5) (H2A, HIST1H2A, H2AFM)
Anti -Histone H2A, acetylated (Lys5) (H2A, HIST1H2A, H2AFM)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces | | | monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs | | | after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3). | | | Catalog # | H5110-08Q1C | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry andImmunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:100,000 | | Immunoprecipitation: 1:70 | | Immunohistochemistry: 1:250-1:500 | | Immunocytochemistry: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H373 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant. | | Immunogen | Synthetic peptide corresponding to residues near the N-terminus of human Histone H2A.. | | Specificity | Recognizes human Histone H2A acetylated at Lysine-5. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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