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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B

Catalog #	H5110-08Q3
Applications	Sutiable for use in Western Blot. Other applications not tested.
Recommended Dilution	Immunoblot Analysis: 1:500-1:20,000 dilution of this lot detected acetylated Histone H2A in sodium butyrate treated acid extracted proteins from HeLa cell.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid containing 0.05% sodium azide and 30% glycerol.
Purity	Whole antiserum immunodepleted with the non-acetylated immunogenic peptide
Immunogen	KLH-conjugated, synthetic peptide (GKQGG[acK]ARAKAK-C) corresponding to aa4-15 of human Histone H2A, to which a C-terminal cysteine was added for conjugation purposes. The immunizing sequence has identical aa in chicken, bovine, rat, mouse and duck.
Specificity	Recognizes acetylated Histone 2A, Mr 14kD. Species Crossreactivity: Human. Broad species crossreactivity expected due to conserved sequence of the immunogen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.