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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A, acetylated (Lys15)

Anti -Histone H2A, acetylated (Lys15)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, creating two nearly symmetrical halves by tertiary structure. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Histone H4 may also be acetylated at Lys5, 8, 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
Catalog #H5110-08Q4
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in liquid PBS, 0.04% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding human Acetyl-Histone H2A (Lys15)(KLH).
SpecificityRecognizes human Acetyl-Histone H2A (Lys15). Species Crossreactivity: mouse, monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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