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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2A.X, gamma, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX)

Anti -Histone H2A.X, gamma, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX,
H2A/X, H2AFX, H2AX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH FC IF
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.
Catalog #H5110-08V4
ApplicationsSuitable for use in Immunofluorescence, Immunohistochemistry, Flow Cytometry and Western Blot. Not recommended for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunofluorescence (IC): 1:500-1:5000
Immunohistochemistry: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Formaldehyde fixation followed by permeabilization with Triton-X 100.
Flow Cytometry: 5ug/1e6 cells in a 150ul reaction
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Positive ControlHuman HEK293 nuclear extracts, breast carcinoma, HeLa cells, ovarian carcinoma, testicular seminoma.
Storage and StabilityStore at 4°C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a region surrounding phosphorylated serine 139 of human histone H2A.X, gamma (GeneID 3014). Cellular localization: Nucleus, chromosomes.
SpecificityRecognizes human histone H2A.X, gamma. Species Crossreactivity: mouse, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGamma H2AX, H2AX, Histone H2AX, Histone H2AFX, Histone H2A.X, Histone H2A/X


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