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Anti -Histone H2A.X, gamma, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX)
Anti -Histone H2A.X, gamma, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX,
H2A/X, H2AFX, H2AX)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IC FC IF |
|
| Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites. | | | Catalog # | H5110-08V4 | | Applications | Suitable for use in Immunofluorescence, Immunocytochemistry, Flow Cytometry and Western Blot. Not recommended for use in Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | Immunocytochemistry: 1:400-1:800 | | Western Blot: 1:5000-1:15,000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human HEK293 nuclear extracts | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide. | | Purity | Purified by affinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to a region surrounding phosphorylated serine 139 of human histone gamma-H2AX (GeneID 3014) | | Specificity | Recognizes human gamma-H2AX when phosphorylated at Ser139. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Gamma H2AX, H2AX, Histone H2AX, Histone H2AFX, Histone H2A.X, Histone H2A/X |
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