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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2B, acetylated (Lys20) (Histone H2B Type 1, H2B-1A, H2B Histone Family, H2B2f, H2Ba, H2Bf, MGC131639)

Anti -Histone H2B, acetylated (Lys20) (Histone H2B Type 1, H2B-1A, H2B Histone Family,
H2B2f, H2Ba, H2Bf, MGC131639)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC IF
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) 2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
Catalog #H5110-09D1
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10
Western Blot: 1:50,000
Immunoprecipitation: 1:10
Immunohistochemistry: 1:250-1:500
Immunocytochemistry/Immunofluorescence: 1:100-1:250 1:250-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlsC6 cell lysate treated or untreated with TSA; Human cervical carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone No9H374
ConcentrationNot detemined
FormSupplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide,
50% glycerol.
ImmunogenSynthetic acetylated peptide corresponding to residues surrounding Lys 20 of Histone H2B.
SpecificityRecognizes human Histone H2B acetylated on Lys 20. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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