| Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code. |
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| Catalog # | H5110-09FN |
| Applications | Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:10,000. Detected phospho-Histone H2B in HL-60 cells treated with 20ug/ml VP16 for 18 hours. A 1:250-1:2000 dilution also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells. |
| Immunocytochemistry: 1:5000 selectively immunostained pycnotic apoptotic HL-60 cells and TUNEL positive HeLa cells. |
| Immunohistochemistry: 1:5000 immunostained deparaffinated Xenopus tail sections. |
| Note: Use of this antibody at dilutions which are too low will result in non-specific detection of Histone H2B and other unknown proteins in immunoblots of acid extracts from untreated cells. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid, 0.05% sodium azide, 30% glycerol. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to aa 6-16, C-SAPAPKKG[pS]KK of human histone H2B (KLH). |
| Specificity | Recognizes hunan phospho-Histone H2B (Ser14), Mr 15kD. Specific for the phosphorylated peptide and does not crossreact with the unphosphorylated counterpart. |
| Species sequence homology | Broad |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
