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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Histones are relatively small proteins that contain a high proportion of positively chargedaa, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Human histone H2B is a 125aa protein.
Catalog #	H5110-09G1
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control	Jurkat, PBMC
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa111-125 AVSEGTKAVTKYTSS of human Histone H2B.
Specificity	Recognizes human Histone H2B. Species Crossreactivity: chicken, canine, drosophila, most mammals, mouse, rat, zebrafish.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.