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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2B, acetylated, aa7-20

Anti -Histone H2B, acetylated, aa7-20

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Catalog #H5110-09K
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000. Detects acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected. Recombinant Histone H2B and 10ug acid-extracted proteins from sodium butyrate treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-09K. Proteins were visualized using a goat anti-rabbit conjugated to HRP secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid with 0.05% sodium azide, 30% glycerol
PuritySerum
ImmunogenSynthetic peptide [APAPKAcKGSKKAITK] corresponding to 7-20aa of human Histone H2B member F. (KLH coupled)
SpecificityRecognizes human acetylated Histone 2B, Mr 15kD. Species Sequence Homology: mouse, chicken and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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