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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B

Catalog #	H5110-09K
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:10,000. Detects acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected. Recombinant Histone H2B and 10ug acid-extracted proteins from sodium butyrate treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-09K. Proteins were visualized using a goat anti-rabbit conjugated to HRP secondary antibody and a chemiluminescence detection system.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
	I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid with 0.05% sodium azide, 30% glycerol
Purity	Serum
Immunogen	Synthetic peptide [APAPKAcKGSKKAITK] corresponding to 7-20aa of human Histone H2B member F. (KLH coupled)
Specificity	Recognizes human acetylated Histone 2B, Mr 15kD. Species Sequence Homology: mouse, chicken and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.