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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2B (NT)

Anti -Histone H2B (NT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
Catalog #H5110-09N
ApplicationsHuman PBMC cell lysate may be used and a 15kD band will be detected. Western blot analysis of Histone-H2B using antibody at 1:500 against human PBMC lysate.
Recommended DilutionWestern blot: 1:500-1:1000
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptides of human Histone-H2B.
SpecificityRecognizes human Histone H2B (NT)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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