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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
Catalog #	H5110-09P
Applications	Suitable for the detection of Histone H2B by Western blot. Human peripheral blood cell lysate or Jurkat cell lysate can be used as positive control. An approximately 15kD band is detected. Western blot analysis of Histone H2B in human PBMC cell lysate with antibody.
Recommended Dilution	Western blot: 2ug/ml
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity	Purified
Immunogen	Synthetic peptide containing amino acid residues 111-125 of human Histone H2B.
Specificity	Recognizes human Histone H2B (CT)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.