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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2B

Anti -Histone H2B

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP IF
Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism.
Catalog #H5110-11
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceBovine
Concentration~2mg/ml
FormSupplied as a liquid in PBS. No preservatives added.
PurityPurified
ImmunogenHistone 2B, calf whole protein complexed to ribosomal RNA.
SpecificityRecognizes bovine Histone H2b: 100%. No reaction with Histone-H-1, Histone-H-3 or Histone-H-4. Very low crossreactivity with Histone-H-core.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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