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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H2B, acetylated (Lys5, Lys12, Lys15, Lys20)

Anti -Histone H2B, acetylated (Lys5, Lys12, Lys15, Lys20)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #H5110-11C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:40,000-1:80,000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide (S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.
SpecificityRecognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat and bovine based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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