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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H2B, acetylated, Lys5

Anti -Histone H2B, acetylated, Lys5

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
Catalog #H5110-11F2
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50,000
Immunohistochemistry: 1:250 500
Immunocytochemistry: 1:250 500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k206
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA
PuritySupernatant
ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B
SpecificityRecognizes human Histone H2B only when acetylated on Lysine-5. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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